Transposon mutagenesis in Staphylococcus epidermidis using the Enterococcus faecalis transposon Tn917
نویسندگان
چکیده
منابع مشابه
Establishment of an arbitrary PCR for rapid identification of Tn917 insertion sites in Staphylococcus epidermidis: characterization of biofilm-negative and nonmucoid mutants.
Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, w...
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The streptococcal transposon Tn917 was demonstrated to transpose in Escherichia coli from the Bacillus subtilis-E. coli shuttle plasmid pHK1207 into an F' plasmid derivative. Subsequently, a second round of transposition from the F' plasmid into pACYC184 could be readily demonstrated. These results represent the initial demonstration of the transposition of a gram-positive transposon in a gram-...
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Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.G., A.P); Institute of Molecular Plant Sciences Leiden University, The Netherlands (P.B.F.O., J.H.C.H); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, France (C.S., E.G.); John Innes Centre, Norwich, United Kingdom (A.K., P.C.); University of Milan, Italy (L.C.); ...
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In order to devise an in vivo insertion mutagenesis scheme for Haemophilus influenzae, a novel set of transposons has been constructed. These are Tn10-based minitransposons carried on pACYC184- and pACYC177-based replicons, which are suitable for in vivo transposition in H. influenzae. The transposon delivery system was designed to contain an H. influenzae-specific uptake signal sequence which ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097
DOI: 10.1016/0378-1097(91)90335-8